Humoral Stimulators of Granulocyte Production

Humoral stimulators of granulocyte production.

These studies were designed to deteractivities followed different patterns. mine whether colony stimulating factor In particular, CSF peaked and was (CSF) and diffusible granulocytopoietic declining 24 hr following endotoxin at stimulator (DGS) represented a single which time DGS was not yet detectsubstance which stimulates the proable. Then, as serum CSF fell, the DGS duction of granulocytes i...

PRODUCTION AND CHARACTERIZATION OF HUMORAL IMMUNE RESPONSE AGAINST MUSTARD GAS

One of the promising aspects of the immunological research on chemical war gas is to investigate the immunogenicity of some hazardous compounds such as mustard gas. Mustard gas is categorized as a "hapten" based on its physical and chemical properties. Haptenic chemicals which do not possess immunogenicity could be immunogenic experimentally when conjugated with a suitable protein carrier...

Humoral regulation of red cell production.

Chlorpromazine inhibition of granulocyte superoxide production.

Superoxide production by granulocytes is a result of the activation of an NAD(P)H-dependent oxidase present in the plasma membrane. Chlorpromazine (5-50 muM) prolongs the time necessary to activation of the superoxide generating system and inhibits the extent of activation. When chlorpromazine is added after activation, there is an inhibition of further superoxide production. These effects are ...

Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...